rabbit anti occludin polyclonal pab Search Results


rabbit anti human serum albumin  (Danaher Inc)
86
Danaher Inc rabbit anti human serum albumin
Rabbit Anti Human Serum Albumin, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti human serum albumin/product/Danaher Inc
Average 86 stars, based on 1 article reviews
rabbit anti human serum albumin - by Bioz Stars, 2026-02
86/100 stars
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house produced rabbit anti cvb1 6 polyclonal antibody  (LI-COR)
86
LI-COR house produced rabbit anti cvb1 6 polyclonal antibody
Characterisation of CVB3-VLPs produced by co-transfection of 3CD and P1. ( A ) SDS-PAGE and Western blot analyses of purified VLPs. The top panel shows the stain-free total protein staining of the purified CVB3-VLPs. The bottom panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit <t>anti-CVB1–6</t> polyclonal antibody. 2 µg of each purified CVB3-VLP was loaded per well recovered from cultures produced as follows (1) 2% 3CD/98% P1, 5 days production, (2) 2% 3CD/98% P1, 8 days production (3) 5% 3CD/95% P1, 5 days production, (4) 5% 3CD/95% P1, 8 days production (5) 10% 3CD/90% P1, 5 days production, 6) 10% 3CD/90% P1, 8 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 5. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs. Transmission electron microscopy images of the sucrose cushion ultracentrifugation purified CVB3-VLPs: ( C ) 2% 3CD/98% P1, 5 days production, ( D ) 2% 3CD/98% P1, 8 days production ( E ) 5% 3CD/95% P1, 5 days production, ( F ) 5% 3CD/95% P1, 8 days production ( G ) 10% 3CD/90% P1, 5 days production, ( H ) 10% 3CD/90% P1, 8 days production Scale bar 200 nm, 50,000 × magnification.
House Produced Rabbit Anti Cvb1 6 Polyclonal Antibody, supplied by LI-COR, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/house produced rabbit anti cvb1 6 polyclonal antibody/product/LI-COR
Average 86 stars, based on 1 article reviews
house produced rabbit anti cvb1 6 polyclonal antibody - by Bioz Stars, 2026-02
86/100 stars
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horseradish peroxidase-conjugated anti-rabbit secondary antibody  (Biokit SA)
90
Biokit SA horseradish peroxidase-conjugated anti-rabbit secondary antibody
Characterisation of CVB3-VLPs produced by co-transfection of 3CD and P1. ( A ) SDS-PAGE and Western blot analyses of purified VLPs. The top panel shows the stain-free total protein staining of the purified CVB3-VLPs. The bottom panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit <t>anti-CVB1–6</t> polyclonal antibody. 2 µg of each purified CVB3-VLP was loaded per well recovered from cultures produced as follows (1) 2% 3CD/98% P1, 5 days production, (2) 2% 3CD/98% P1, 8 days production (3) 5% 3CD/95% P1, 5 days production, (4) 5% 3CD/95% P1, 8 days production (5) 10% 3CD/90% P1, 5 days production, 6) 10% 3CD/90% P1, 8 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 5. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs. Transmission electron microscopy images of the sucrose cushion ultracentrifugation purified CVB3-VLPs: ( C ) 2% 3CD/98% P1, 5 days production, ( D ) 2% 3CD/98% P1, 8 days production ( E ) 5% 3CD/95% P1, 5 days production, ( F ) 5% 3CD/95% P1, 8 days production ( G ) 10% 3CD/90% P1, 5 days production, ( H ) 10% 3CD/90% P1, 8 days production Scale bar 200 nm, 50,000 × magnification.
Horseradish Peroxidase Conjugated Anti Rabbit Secondary Antibody, supplied by Biokit SA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/horseradish peroxidase-conjugated anti-rabbit secondary antibody/product/Biokit SA
Average 90 stars, based on 1 article reviews
horseradish peroxidase-conjugated anti-rabbit secondary antibody - by Bioz Stars, 2026-02
90/100 stars
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rabbit anti-psma1 pab (a3460, proteasomes)  (ABclonal Biotechnology)
90
ABclonal Biotechnology rabbit anti-psma1 pab (a3460, proteasomes)
Characterisation of CVB3-VLPs produced by co-transfection of 3CD and P1. ( A ) SDS-PAGE and Western blot analyses of purified VLPs. The top panel shows the stain-free total protein staining of the purified CVB3-VLPs. The bottom panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit <t>anti-CVB1–6</t> polyclonal antibody. 2 µg of each purified CVB3-VLP was loaded per well recovered from cultures produced as follows (1) 2% 3CD/98% P1, 5 days production, (2) 2% 3CD/98% P1, 8 days production (3) 5% 3CD/95% P1, 5 days production, (4) 5% 3CD/95% P1, 8 days production (5) 10% 3CD/90% P1, 5 days production, 6) 10% 3CD/90% P1, 8 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 5. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs. Transmission electron microscopy images of the sucrose cushion ultracentrifugation purified CVB3-VLPs: ( C ) 2% 3CD/98% P1, 5 days production, ( D ) 2% 3CD/98% P1, 8 days production ( E ) 5% 3CD/95% P1, 5 days production, ( F ) 5% 3CD/95% P1, 8 days production ( G ) 10% 3CD/90% P1, 5 days production, ( H ) 10% 3CD/90% P1, 8 days production Scale bar 200 nm, 50,000 × magnification.
Rabbit Anti Psma1 Pab (A3460, Proteasomes), supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-psma1 pab (a3460, proteasomes)/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
rabbit anti-psma1 pab (a3460, proteasomes) - by Bioz Stars, 2026-02
90/100 stars
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antibody rabbit polyclonal anti-keratin 15  (ABclonal Biotechnology)
90
ABclonal Biotechnology antibody rabbit polyclonal anti-keratin 15
Characterisation of CVB3-VLPs produced by co-transfection of 3CD and P1. ( A ) SDS-PAGE and Western blot analyses of purified VLPs. The top panel shows the stain-free total protein staining of the purified CVB3-VLPs. The bottom panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit <t>anti-CVB1–6</t> polyclonal antibody. 2 µg of each purified CVB3-VLP was loaded per well recovered from cultures produced as follows (1) 2% 3CD/98% P1, 5 days production, (2) 2% 3CD/98% P1, 8 days production (3) 5% 3CD/95% P1, 5 days production, (4) 5% 3CD/95% P1, 8 days production (5) 10% 3CD/90% P1, 5 days production, 6) 10% 3CD/90% P1, 8 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 5. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs. Transmission electron microscopy images of the sucrose cushion ultracentrifugation purified CVB3-VLPs: ( C ) 2% 3CD/98% P1, 5 days production, ( D ) 2% 3CD/98% P1, 8 days production ( E ) 5% 3CD/95% P1, 5 days production, ( F ) 5% 3CD/95% P1, 8 days production ( G ) 10% 3CD/90% P1, 5 days production, ( H ) 10% 3CD/90% P1, 8 days production Scale bar 200 nm, 50,000 × magnification.
Antibody Rabbit Polyclonal Anti Keratin 15, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody rabbit polyclonal anti-keratin 15/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
antibody rabbit polyclonal anti-keratin 15 - by Bioz Stars, 2026-02
90/100 stars
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rabbit polyclonal bcl-xl antibody clone arc0183  (ABclonal Biotechnology)
90
ABclonal Biotechnology rabbit polyclonal bcl-xl antibody clone arc0183
Characterisation of CVB3-VLPs produced by co-transfection of 3CD and P1. ( A ) SDS-PAGE and Western blot analyses of purified VLPs. The top panel shows the stain-free total protein staining of the purified CVB3-VLPs. The bottom panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit <t>anti-CVB1–6</t> polyclonal antibody. 2 µg of each purified CVB3-VLP was loaded per well recovered from cultures produced as follows (1) 2% 3CD/98% P1, 5 days production, (2) 2% 3CD/98% P1, 8 days production (3) 5% 3CD/95% P1, 5 days production, (4) 5% 3CD/95% P1, 8 days production (5) 10% 3CD/90% P1, 5 days production, 6) 10% 3CD/90% P1, 8 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 5. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs. Transmission electron microscopy images of the sucrose cushion ultracentrifugation purified CVB3-VLPs: ( C ) 2% 3CD/98% P1, 5 days production, ( D ) 2% 3CD/98% P1, 8 days production ( E ) 5% 3CD/95% P1, 5 days production, ( F ) 5% 3CD/95% P1, 8 days production ( G ) 10% 3CD/90% P1, 5 days production, ( H ) 10% 3CD/90% P1, 8 days production Scale bar 200 nm, 50,000 × magnification.
Rabbit Polyclonal Bcl Xl Antibody Clone Arc0183, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal bcl-xl antibody clone arc0183/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
rabbit polyclonal bcl-xl antibody clone arc0183 - by Bioz Stars, 2026-02
90/100 stars
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glucocorticoid receptor (gr) rabbit pab  (ABclonal Biotechnology)
90
ABclonal Biotechnology glucocorticoid receptor (gr) rabbit pab
Characterisation of CVB3-VLPs produced by co-transfection of 3CD and P1. ( A ) SDS-PAGE and Western blot analyses of purified VLPs. The top panel shows the stain-free total protein staining of the purified CVB3-VLPs. The bottom panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit <t>anti-CVB1–6</t> polyclonal antibody. 2 µg of each purified CVB3-VLP was loaded per well recovered from cultures produced as follows (1) 2% 3CD/98% P1, 5 days production, (2) 2% 3CD/98% P1, 8 days production (3) 5% 3CD/95% P1, 5 days production, (4) 5% 3CD/95% P1, 8 days production (5) 10% 3CD/90% P1, 5 days production, 6) 10% 3CD/90% P1, 8 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 5. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs. Transmission electron microscopy images of the sucrose cushion ultracentrifugation purified CVB3-VLPs: ( C ) 2% 3CD/98% P1, 5 days production, ( D ) 2% 3CD/98% P1, 8 days production ( E ) 5% 3CD/95% P1, 5 days production, ( F ) 5% 3CD/95% P1, 8 days production ( G ) 10% 3CD/90% P1, 5 days production, ( H ) 10% 3CD/90% P1, 8 days production Scale bar 200 nm, 50,000 × magnification.
Glucocorticoid Receptor (Gr) Rabbit Pab, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glucocorticoid receptor (gr) rabbit pab/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
glucocorticoid receptor (gr) rabbit pab - by Bioz Stars, 2026-02
90/100 stars
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anti-rabbit igg hrβ-conjugate  (Promega)
90
Promega anti-rabbit igg hrβ-conjugate
Characterisation of CVB3-VLPs produced by co-transfection of 3CD and P1. ( A ) SDS-PAGE and Western blot analyses of purified VLPs. The top panel shows the stain-free total protein staining of the purified CVB3-VLPs. The bottom panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit <t>anti-CVB1–6</t> polyclonal antibody. 2 µg of each purified CVB3-VLP was loaded per well recovered from cultures produced as follows (1) 2% 3CD/98% P1, 5 days production, (2) 2% 3CD/98% P1, 8 days production (3) 5% 3CD/95% P1, 5 days production, (4) 5% 3CD/95% P1, 8 days production (5) 10% 3CD/90% P1, 5 days production, 6) 10% 3CD/90% P1, 8 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 5. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs. Transmission electron microscopy images of the sucrose cushion ultracentrifugation purified CVB3-VLPs: ( C ) 2% 3CD/98% P1, 5 days production, ( D ) 2% 3CD/98% P1, 8 days production ( E ) 5% 3CD/95% P1, 5 days production, ( F ) 5% 3CD/95% P1, 8 days production ( G ) 10% 3CD/90% P1, 5 days production, ( H ) 10% 3CD/90% P1, 8 days production Scale bar 200 nm, 50,000 × magnification.
Anti Rabbit Igg Hrβ Conjugate, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-rabbit igg hrβ-conjugate/product/Promega
Average 90 stars, based on 1 article reviews
anti-rabbit igg hrβ-conjugate - by Bioz Stars, 2026-02
90/100 stars
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rabbit polyclonal anti-slitrk4  (Promega)
90
Promega rabbit polyclonal anti-slitrk4
Characterisation of CVB3-VLPs produced by co-transfection of 3CD and P1. ( A ) SDS-PAGE and Western blot analyses of purified VLPs. The top panel shows the stain-free total protein staining of the purified CVB3-VLPs. The bottom panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit <t>anti-CVB1–6</t> polyclonal antibody. 2 µg of each purified CVB3-VLP was loaded per well recovered from cultures produced as follows (1) 2% 3CD/98% P1, 5 days production, (2) 2% 3CD/98% P1, 8 days production (3) 5% 3CD/95% P1, 5 days production, (4) 5% 3CD/95% P1, 8 days production (5) 10% 3CD/90% P1, 5 days production, 6) 10% 3CD/90% P1, 8 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 5. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs. Transmission electron microscopy images of the sucrose cushion ultracentrifugation purified CVB3-VLPs: ( C ) 2% 3CD/98% P1, 5 days production, ( D ) 2% 3CD/98% P1, 8 days production ( E ) 5% 3CD/95% P1, 5 days production, ( F ) 5% 3CD/95% P1, 8 days production ( G ) 10% 3CD/90% P1, 5 days production, ( H ) 10% 3CD/90% P1, 8 days production Scale bar 200 nm, 50,000 × magnification.
Rabbit Polyclonal Anti Slitrk4, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti-slitrk4/product/Promega
Average 90 stars, based on 1 article reviews
rabbit polyclonal anti-slitrk4 - by Bioz Stars, 2026-02
90/100 stars
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hrp-goat anti-rabbit antibody hs101  (Yeasen Biotechnology)
90
Yeasen Biotechnology hrp-goat anti-rabbit antibody hs101
Characterisation of CVB3-VLPs produced by co-transfection of 3CD and P1. ( A ) SDS-PAGE and Western blot analyses of purified VLPs. The top panel shows the stain-free total protein staining of the purified CVB3-VLPs. The bottom panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit <t>anti-CVB1–6</t> polyclonal antibody. 2 µg of each purified CVB3-VLP was loaded per well recovered from cultures produced as follows (1) 2% 3CD/98% P1, 5 days production, (2) 2% 3CD/98% P1, 8 days production (3) 5% 3CD/95% P1, 5 days production, (4) 5% 3CD/95% P1, 8 days production (5) 10% 3CD/90% P1, 5 days production, 6) 10% 3CD/90% P1, 8 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 5. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs. Transmission electron microscopy images of the sucrose cushion ultracentrifugation purified CVB3-VLPs: ( C ) 2% 3CD/98% P1, 5 days production, ( D ) 2% 3CD/98% P1, 8 days production ( E ) 5% 3CD/95% P1, 5 days production, ( F ) 5% 3CD/95% P1, 8 days production ( G ) 10% 3CD/90% P1, 5 days production, ( H ) 10% 3CD/90% P1, 8 days production Scale bar 200 nm, 50,000 × magnification.
Hrp Goat Anti Rabbit Antibody Hs101, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrp-goat anti-rabbit antibody hs101/product/Yeasen Biotechnology
Average 90 stars, based on 1 article reviews
hrp-goat anti-rabbit antibody hs101 - by Bioz Stars, 2026-02
90/100 stars
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rabbit anti-ling zhi immunomodulatory protein serum  (Taiwan Advance Bio)
90
Taiwan Advance Bio rabbit anti-ling zhi immunomodulatory protein serum
Characterisation of CVB3-VLPs produced by co-transfection of 3CD and P1. ( A ) SDS-PAGE and Western blot analyses of purified VLPs. The top panel shows the stain-free total protein staining of the purified CVB3-VLPs. The bottom panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit <t>anti-CVB1–6</t> polyclonal antibody. 2 µg of each purified CVB3-VLP was loaded per well recovered from cultures produced as follows (1) 2% 3CD/98% P1, 5 days production, (2) 2% 3CD/98% P1, 8 days production (3) 5% 3CD/95% P1, 5 days production, (4) 5% 3CD/95% P1, 8 days production (5) 10% 3CD/90% P1, 5 days production, 6) 10% 3CD/90% P1, 8 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 5. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs. Transmission electron microscopy images of the sucrose cushion ultracentrifugation purified CVB3-VLPs: ( C ) 2% 3CD/98% P1, 5 days production, ( D ) 2% 3CD/98% P1, 8 days production ( E ) 5% 3CD/95% P1, 5 days production, ( F ) 5% 3CD/95% P1, 8 days production ( G ) 10% 3CD/90% P1, 5 days production, ( H ) 10% 3CD/90% P1, 8 days production Scale bar 200 nm, 50,000 × magnification.
Rabbit Anti Ling Zhi Immunomodulatory Protein Serum, supplied by Taiwan Advance Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-ling zhi immunomodulatory protein serum/product/Taiwan Advance Bio
Average 90 stars, based on 1 article reviews
rabbit anti-ling zhi immunomodulatory protein serum - by Bioz Stars, 2026-02
90/100 stars
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acat1  (ELK Biotechnology)
90
ELK Biotechnology acat1
( A and E ) DCA evaluating the prediction efficacy of the nomogram in the testing ( A ) and validation sets ( E ). ( B and F ) Calibration curves evaluating the clinical benefit of the nomogram in the testing ( B ) and validation sets ( F ). ( C and G ) ROC evaluation of the nomogram’s diagnostic performance in the testing ( C ) and validation sets ( G ). ( D and H ) Risk score derived from the model for patients with UC and HC in the testing ( D ) and validation sets ( H ). ( I ) ROC curve analysis for <t>ACAT1,</t> ACOX2, and HADHB in diagnosing UC in the external dataset. ( J ) Expression levels of ACAT1, ACOX2, and HADHB in patients with UC and HC in the external dataset.
Acat1, supplied by ELK Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acat1/product/ELK Biotechnology
Average 90 stars, based on 1 article reviews
acat1 - by Bioz Stars, 2026-02
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Image Search Results


Characterisation of CVB3-VLPs produced by co-transfection of 3CD and P1. ( A ) SDS-PAGE and Western blot analyses of purified VLPs. The top panel shows the stain-free total protein staining of the purified CVB3-VLPs. The bottom panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit anti-CVB1–6 polyclonal antibody. 2 µg of each purified CVB3-VLP was loaded per well recovered from cultures produced as follows (1) 2% 3CD/98% P1, 5 days production, (2) 2% 3CD/98% P1, 8 days production (3) 5% 3CD/95% P1, 5 days production, (4) 5% 3CD/95% P1, 8 days production (5) 10% 3CD/90% P1, 5 days production, 6) 10% 3CD/90% P1, 8 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 5. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs. Transmission electron microscopy images of the sucrose cushion ultracentrifugation purified CVB3-VLPs: ( C ) 2% 3CD/98% P1, 5 days production, ( D ) 2% 3CD/98% P1, 8 days production ( E ) 5% 3CD/95% P1, 5 days production, ( F ) 5% 3CD/95% P1, 8 days production ( G ) 10% 3CD/90% P1, 5 days production, ( H ) 10% 3CD/90% P1, 8 days production Scale bar 200 nm, 50,000 × magnification.

Journal: Scientific Reports

Article Title: Production of norovirus-, rotavirus-, and enterovirus-like particles in insect cells is simplified by plasmid-based expression

doi: 10.1038/s41598-024-65316-6

Figure Lengend Snippet: Characterisation of CVB3-VLPs produced by co-transfection of 3CD and P1. ( A ) SDS-PAGE and Western blot analyses of purified VLPs. The top panel shows the stain-free total protein staining of the purified CVB3-VLPs. The bottom panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit anti-CVB1–6 polyclonal antibody. 2 µg of each purified CVB3-VLP was loaded per well recovered from cultures produced as follows (1) 2% 3CD/98% P1, 5 days production, (2) 2% 3CD/98% P1, 8 days production (3) 5% 3CD/95% P1, 5 days production, (4) 5% 3CD/95% P1, 8 days production (5) 10% 3CD/90% P1, 5 days production, 6) 10% 3CD/90% P1, 8 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 5. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs. Transmission electron microscopy images of the sucrose cushion ultracentrifugation purified CVB3-VLPs: ( C ) 2% 3CD/98% P1, 5 days production, ( D ) 2% 3CD/98% P1, 8 days production ( E ) 5% 3CD/95% P1, 5 days production, ( F ) 5% 3CD/95% P1, 8 days production ( G ) 10% 3CD/90% P1, 5 days production, ( H ) 10% 3CD/90% P1, 8 days production Scale bar 200 nm, 50,000 × magnification.

Article Snippet: VP1 and VP3 proteins were detected by Western blotting using in-house produced rabbit anti-CVB1-6 polyclonal antibody and IRDye-labelled secondary antibody (LI-COR Biosciences).

Techniques: Produced, Cotransfection, SDS Page, Western Blot, Purification, Staining, Transmission Assay, Electron Microscopy

Characterisation of CVB3-VLP after pilot-scale production and ion-exchange purification. ( A ) SDS-PAGE and Western blot analyses of the purified VLPs. The left panel shows the total protein staining of the purified CVB3-VLPs with stain-free method. The right panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit anti-CVB1-6 polyclonal antibody. 2 µg purified CVB3-VLP was loaded per well produced as follows (1) 10% 3CD/90% P1, 8 days production, (2) BEVS, 5 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 6. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs from BEVS or plasmid-based production followed by tangential flow filtration and ion exchange chromatography purification ( C ) representative transmission electron microscopy image of purified CVB3-VLP from plasmid-based expression system. 50,000 × magnification, scale bar 200 nm.

Journal: Scientific Reports

Article Title: Production of norovirus-, rotavirus-, and enterovirus-like particles in insect cells is simplified by plasmid-based expression

doi: 10.1038/s41598-024-65316-6

Figure Lengend Snippet: Characterisation of CVB3-VLP after pilot-scale production and ion-exchange purification. ( A ) SDS-PAGE and Western blot analyses of the purified VLPs. The left panel shows the total protein staining of the purified CVB3-VLPs with stain-free method. The right panel shows VP0, VP1 and VP3 capsid protein detection by Western blot using an in-house produced rabbit anti-CVB1-6 polyclonal antibody. 2 µg purified CVB3-VLP was loaded per well produced as follows (1) 10% 3CD/90% P1, 8 days production, (2) BEVS, 5 days production. The gel and blot were cropped for clarity. For full photos, see Supplementary Fig. 6. ( B ) Dynamic light scattering analysis of the purified CVB3-VLPs from BEVS or plasmid-based production followed by tangential flow filtration and ion exchange chromatography purification ( C ) representative transmission electron microscopy image of purified CVB3-VLP from plasmid-based expression system. 50,000 × magnification, scale bar 200 nm.

Article Snippet: VP1 and VP3 proteins were detected by Western blotting using in-house produced rabbit anti-CVB1-6 polyclonal antibody and IRDye-labelled secondary antibody (LI-COR Biosciences).

Techniques: Purification, SDS Page, Western Blot, Staining, Produced, Plasmid Preparation, Filtration, Ion Exchange Chromatography, Transmission Assay, Electron Microscopy, Expressing

( A and E ) DCA evaluating the prediction efficacy of the nomogram in the testing ( A ) and validation sets ( E ). ( B and F ) Calibration curves evaluating the clinical benefit of the nomogram in the testing ( B ) and validation sets ( F ). ( C and G ) ROC evaluation of the nomogram’s diagnostic performance in the testing ( C ) and validation sets ( G ). ( D and H ) Risk score derived from the model for patients with UC and HC in the testing ( D ) and validation sets ( H ). ( I ) ROC curve analysis for ACAT1, ACOX2, and HADHB in diagnosing UC in the external dataset. ( J ) Expression levels of ACAT1, ACOX2, and HADHB in patients with UC and HC in the external dataset.

Journal: Journal of Inflammation Research

Article Title: Unveiling and Validating the Role of Fatty Acid Metabolism in Ulcerative Colitis

doi: 10.2147/JIR.S479011

Figure Lengend Snippet: ( A and E ) DCA evaluating the prediction efficacy of the nomogram in the testing ( A ) and validation sets ( E ). ( B and F ) Calibration curves evaluating the clinical benefit of the nomogram in the testing ( B ) and validation sets ( F ). ( C and G ) ROC evaluation of the nomogram’s diagnostic performance in the testing ( C ) and validation sets ( G ). ( D and H ) Risk score derived from the model for patients with UC and HC in the testing ( D ) and validation sets ( H ). ( I ) ROC curve analysis for ACAT1, ACOX2, and HADHB in diagnosing UC in the external dataset. ( J ) Expression levels of ACAT1, ACOX2, and HADHB in patients with UC and HC in the external dataset.

Article Snippet: The antibodies used included HADHB (Rabbit, 1:2000, Proteintech, China), ACOX2 (Rabbit, 1:1000, ELK Biotechnology), and ACAT1 (Rabbit, 1:1000, ELK Biotechnology).

Techniques: Diagnostic Assay, Derivative Assay, Expressing

Validation of gene expression in colon tissues. ( A and B ) Relative weight change ( A ) and disease activity index ( B ) in each group ( n = 6). ( C ) Representative morphological images of mouse colons from the control and DSS groups. ( D ) Statistical analysis of colon length. ( E ) Representative H&E staining of colonic tissues from each group. ( F ) Statistical results for histological damage scores in each group ( n = 6). ( G ) Western blot analysis of ACAT1, ACOX2, and HADHB levels in colon tissues. Group identifications are as shown. ( H–J ) Quantitative analysis of Western blot results for ACAT1 ( H ), ACOX2 ( I ), and HADHB ( J ) expression. Data are presented as mean ± SEM.

Journal: Journal of Inflammation Research

Article Title: Unveiling and Validating the Role of Fatty Acid Metabolism in Ulcerative Colitis

doi: 10.2147/JIR.S479011

Figure Lengend Snippet: Validation of gene expression in colon tissues. ( A and B ) Relative weight change ( A ) and disease activity index ( B ) in each group ( n = 6). ( C ) Representative morphological images of mouse colons from the control and DSS groups. ( D ) Statistical analysis of colon length. ( E ) Representative H&E staining of colonic tissues from each group. ( F ) Statistical results for histological damage scores in each group ( n = 6). ( G ) Western blot analysis of ACAT1, ACOX2, and HADHB levels in colon tissues. Group identifications are as shown. ( H–J ) Quantitative analysis of Western blot results for ACAT1 ( H ), ACOX2 ( I ), and HADHB ( J ) expression. Data are presented as mean ± SEM.

Article Snippet: The antibodies used included HADHB (Rabbit, 1:2000, Proteintech, China), ACOX2 (Rabbit, 1:1000, ELK Biotechnology), and ACAT1 (Rabbit, 1:1000, ELK Biotechnology).

Techniques: Expressing, Activity Assay, Control, Staining, Western Blot

( A ) Heatmap illustrating variations in immune cell infiltration in the colon tissues of patients with UC and HC. ( B ) Differences in infiltrated immune cells between patients with UC and HC. ( C ) Heatmap showing correlations among immune cells. ( D–F ) Lollipop charts presenting the correlations between ACAT1 ( D ), ACOX2 ( E ), HADHB ( F ) expression, and immune cells.

Journal: Journal of Inflammation Research

Article Title: Unveiling and Validating the Role of Fatty Acid Metabolism in Ulcerative Colitis

doi: 10.2147/JIR.S479011

Figure Lengend Snippet: ( A ) Heatmap illustrating variations in immune cell infiltration in the colon tissues of patients with UC and HC. ( B ) Differences in infiltrated immune cells between patients with UC and HC. ( C ) Heatmap showing correlations among immune cells. ( D–F ) Lollipop charts presenting the correlations between ACAT1 ( D ), ACOX2 ( E ), HADHB ( F ) expression, and immune cells.

Article Snippet: The antibodies used included HADHB (Rabbit, 1:2000, Proteintech, China), ACOX2 (Rabbit, 1:1000, ELK Biotechnology), and ACAT1 (Rabbit, 1:1000, ELK Biotechnology).

Techniques: Expressing

Identification and enrichment analysis of fatty acid metabolism-related subtypes. ( A ) Consensus clustering matrix at k = 2. ( B ) Consensus scores for each subtype at k = 2 to 6. ( C ) PCA plot distinguishing subtype 1 (blue) from subtype 2 (red) samples. ( D ) Split violin plot showing the expression of ACAT1, ACOX2, and HADHB between subtypes. ( E and F ) Volcano plot ( E ) and heatmap ( F ) depicting gene expression patterns between subtypes.

Journal: Journal of Inflammation Research

Article Title: Unveiling and Validating the Role of Fatty Acid Metabolism in Ulcerative Colitis

doi: 10.2147/JIR.S479011

Figure Lengend Snippet: Identification and enrichment analysis of fatty acid metabolism-related subtypes. ( A ) Consensus clustering matrix at k = 2. ( B ) Consensus scores for each subtype at k = 2 to 6. ( C ) PCA plot distinguishing subtype 1 (blue) from subtype 2 (red) samples. ( D ) Split violin plot showing the expression of ACAT1, ACOX2, and HADHB between subtypes. ( E and F ) Volcano plot ( E ) and heatmap ( F ) depicting gene expression patterns between subtypes.

Article Snippet: The antibodies used included HADHB (Rabbit, 1:2000, Proteintech, China), ACOX2 (Rabbit, 1:1000, ELK Biotechnology), and ACAT1 (Rabbit, 1:1000, ELK Biotechnology).

Techniques: Expressing